Intern- Process Development, DNA Development

AskBio Inc. a wholly owned and independently operated subsidiary of Bayer AG is a fully integrated gene therapy company dedicated to developing life-saving medicines and changing lives. The company maintains a portfolio of clinical programs across a range of neuromuscular central nervous system cardiovascular and metabolic disease indications with a clinical-stage pipeline that includes investigational therapeutics for congestive heart failure limb-girdle muscular dystrophy multiple system atrophy Parkinsons disease and Pompe disease. AskBios gene therapy platform includes Pro10 an industry-leading proprietary cell line manufacturing process and an extensive array of capsids and promoters. With global headquarters in Research Triangle Park North Carolina and European headquarters in Edinburgh Scotland the company has generated hundreds of proprietary capsids and promoters several of which have entered pre-clinical and clinical testing.

Our vision: Pioneering science to create transformative molecular medicines.

Our mission: Lead innovative science and drive clinical outcomes to transform peoples lives.

Our principles:

• Advance innovative science by pushing boundaries.

• Bring transformative therapeutics to patients in need.

• Provide an environment for employees to reach their fullest potential.

Our values:

• Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need.

• Cultivate Collaboration. Strive to be the best teammate actively listen openly communicate and embrace diverse points of view.

• Embrace Responsibility. We are humbled by the enormity of our hold a relentless commitment to advance science and clinical outcomes for our patients families and caregivers.

• Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action.

• Act with Uncompromising Integrity. Be honest transparent and committed to doing whats right in every situation. Make clear commitments and follow through.

Position Summary

The purpose of the Process Development DNA Development Intern position is to support the DNA Development team in ongoing development activities. This project will contribute to evaluating the impact of Inverted Terminal Repeats stability on rAAV manufacturing. This role offers hands-on experience in a laboratory and exposure to key elements of molecular biology and plasmid production.

This position is based in RTP NC and will report to the Associate Director DNA Development.

Job Responsibilities

• Learn & support plasmid constructs generation including cloning strategy transformation colony screening and establishing bacterial glycerol stocks.

• Perform bacterial culture growth to support & optimize plasmid production process.

• Purify plasmid DNA from bacterial cultures using established laboratory methods.

• Communicate experimental observations and progress regularly with hiring manager and project team.

• Prepare and deliver a final presentation summarizing project outcomes at the end of the internship.

• Operate within the team structure working under the direct guidance of an assigned mentor and reporting progress to the hiring manager.

Minimum Requirements

• Must be enrolled in Bachelors degree program in a relevant field (Molecular Biology Biotechnology Chemistry/Biochemistry Chemical/Biochemical Engineering or equivalent)

• Must be at least a junior at university at the time of your application

• Should have a basic understanding of one or more of the following techniques: aseptic technique making reagents at desired dilutions or concentrations Restriction Enzyme digest Gel Electrophoresis DNA quantitation identifying annotated features on a plasmid map bacterial cell culture centrifugation filtration and liquid chromatography

• Strong attention to detail highly organized and the ability to positively interact in a team environment

• Excellent verbal and written communication skills with the ability to maintain formal record keeping

• Comfortable with working and learning in a fast-paced laboratory environment

• Service-oriented and quality mindset/attitude

• Proficiency in Microsoft Office Suite (Word Outlook Excel)

Preferred Education Experience and Skills

• Prior internship work or volunteer/extracurricular experience

• Prior experience working in a lab environment

• Experience with SnapGene software

AskBio Inc. (AskBio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race color religion gender sexual orientation gender identity national origin age disability veteran status or any other protected status prohibited under Federal State or local laws. All employment decisions are based on valid jobrelated requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website you may request a reasonable accommodation to express interest in a specific opening by calling us at or sending us an email at .

Agencies: Please do not contact any employee at AskBio about this requisition. Any resume submitted by a recruitment agency to any employee at AskBio through any medium will be deemed the sole property of AskBio unless the agency was engaged by AskBio Talent Acquisition team to recruit for that position. All agencies must have a prior executed service agreement with AskBio prior to any search engagement. If a candidate who was submitted outside of the AskBio agency process is hired by AskBio no fee or payment of any kind will be paid to the agency.